Prerequisites

• image_pixel_content

Image display

brightness = ( value - min ) / ( max - min )

if ( brightness < 0 ) set to zero
if ( brightness > 1 ) set to one

0 <= brightness <= 1

contrast = max - min

Activity

• Open image: xy_16bit__nuclei_high_dynamic_range.tif
• Explore different single color LUTs and LUT settings
• Appreciate that LUT settings do not change the pixel values
• Explore multi-color LUT for visualisation of high dynamic range images
  • Add a LUT calibration bar to the image

Formative Assessment

Fill in the blanks

Fill in the blanks, using those words: decrease, larger than, increase, smaller than

1. Pixels with values _____ max will appear saturated.
2. Decreasing max while keeping min constant will _____ the contrast.
3. Decreasing both max and min will _____ the overall brightness.
4. Pixels with values _____ the min will appear black, when using a grayscale LUT.

TODO: Comparative image display

Open two images and display with the same LUT settings.