Object splitting

Requirements

• binarisation.md
• distance_transform.md

“Intensity based” watershed

Activity: Explore intensity based watershed

• Open image: xy_8bit__touching_objects.tif
• Invert image for watershed
• Apply watershed

Activity: Use intensity based watershed for object segmentation

• Open intensity image: xy_8bit__touching_objects.tif
• Threshold intensity image => binary image (aka “mask”)
• Invert intensity image for watershed
• Apply watershed, using the mask

“Shape based” watershed

Activity: Explore shape based watershed

• Open image: xy_8bit__touching_objects_same_intensity.tif
• Threshold -> Binary image
  • Copy binary image (we’ll need it as mask later…)
• Binary image -> Distance map
• Distance map -> Watershed

Learn more

TODO

Formative Assessment

TODO